Letter from Daniel J. Minnema to Marshall W. Nirenberg
Minnema, from the Department of Environmental Health at the University of Cincinnati Medical Center, asks Nirenberg for access
to cell lines for examining the effects of chronic toxicant exposure on nerve cells.
I recently read an article in Brain Research by Dr. Monsma et al. in which they characterize D1 and D2 dopamine receptors
in neuroblastoma cell lines. One important use of this cell line is for the in vitro testing of neurotoxicants that potentially
affect dopamine receptors. Dr. Monsma has referred me to you as the supplier of the NS20Y cells. I would kindly appreciate
obtaining some of these NS20Y cells as a generous gift to initiate a colony here at the University of Cincinnati, Department
of Environmental Health, specifically for examining the effects of "chronic" toxicant exposure on such nerve cells.
In addition I would need the appropriate information as to any special requirements necessary to maintain these cells in culture.
We will not pass the cell line on to anyone, and would make you aware of any results obtained with the cell line. Of course,
I am more than willing to pay the necessary expenses for transportation and handling. Thank you in advance.