Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver
Prior studies to this article by Anfinsen, Epstein, and Goldberger established that the time required for synthesizing a chain
of protein such as ribonuclease in the tissues of higher organisms would be approximately two minutes. Here, the three researchers
noted that an enzyme system in the endoplasmic reticulum of cells that catalyzed the disulfide interchange reaction and which,
when added to solutions of reduced ribonuclease, catalyzed the rapid formation of the native disulfide pairing in a period
less than two minutes. Subsequent studies would find that such motile proteins as staphylococcal nuclease or myoglobin could
undergo virtually complete renaturation in only a few seconds or less.
Number of Image Pages:
8 (1,308,970 Bytes)
1963-02 (February 1963)
Anfinsen, Christian B.
Periodical: Goldberger, Robert, Charles Epstein, and Christian B. Anfinsen. "Acceleration of Reactivation of Reduced Bovine Pancreatic
Ribonuclease by a Microsomal System from Rat Liver." Journal of Biological Chemistry 238, 2 (February 1963): 628-635.
Article. 8 Images.
American Society for Biochemistry and Molecular Biology
Reproduced with permission of the American Society for Biochemistry and Molecular Biology.