The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease
In this article, Anfinsen's team from the Laboratory of Chemical Biology at the NIAMD became the first to report that
they had determined the total amino acid sequence of an extracellular nuclease of Staphylococcus aureus, which consisted of
a single chain of 149 amino acid residues. They also noted that organic synthesis of the polypeptide chain of the nuclease
would be of value in the direct study of catalytic and binding properties, and of factors determining tertiary structure.
The proposed plan for synthesis involved assembling the chain through successive couplings of protected peptide fragments
to the free alpha-amino group of protected C-terminal sequence. In this article, residue 43 was mistakenly identified as
glutamine, instead of glutamic acid; A subsequent article would correct this error.
Number of Image Pages:
6 (666,740 Bytes)
1967-10 (October 1967)
Anfinsen, Christian B.
Periodical: Anfinsen, Christian B., David Ontjes, Motonori Ohno, Lila Corley, and Ann Eastlake. "The Synthesis of Protected Peptide
Fragments of a Staphylococcal Nuclease." Proceedings of the National Academy of Sciences of the United States of America
58, 4 (October 1967): 1806-1811. Article. 6 Images.
National Academy Press
Reproduced with permission of Libby Anfinsen.
Medical Subject Headings (MeSH):
Amino Acid Sequence
Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme (October 1969)