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The Christian B. Anfinsen Papers

Title:
The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease pdf (666,740 Bytes) ocr (20,056 Bytes)
The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease
Description:
In this article, Anfinsen's team from the Laboratory of Chemical Biology at the NIAMD became the first to report that they had determined the total amino acid sequence of an extracellular nuclease of Staphylococcus aureus, which consisted of a single chain of 149 amino acid residues. They also noted that organic synthesis of the polypeptide chain of the nuclease would be of value in the direct study of catalytic and binding properties, and of factors determining tertiary structure. The proposed plan for synthesis involved assembling the chain through successive couplings of protected peptide fragments to the free alpha-amino group of protected C-terminal sequence. In this article, residue 43 was mistakenly identified as glutamine, instead of glutamic acid; A subsequent article would correct this error.
Number of Image Pages:
6 (666,740 Bytes)
Date:
1967-10 (October 1967)
Creator:
Anfinsen, Christian B.
Ontjes, David
Ohno, Motonori
Corley, Lila
Eastlake, Ann
Source:
Periodical: Anfinsen, Christian B., David Ontjes, Motonori Ohno, Lila Corley, and Ann Eastlake. "The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease." Proceedings of the National Academy of Sciences of the United States of America 58, 4 (October 1967): 1806-1811. Article. 6 Images.
Publisher:
National Academy Press
Rights:
Reproduced with permission of Libby Anfinsen.
Subject:
Medical Subject Headings (MeSH):
Amino Acid Sequence
Staphylococcus aureus
Research Design
Relation:
Metadata Record Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme (October 1969) pdf (946,143 Bytes) ocr (25,088 Bytes)
/ps/access/KKBBLH.pdf
Box Number: 27
Folder Number: 10
Unique Identifier:
KKBBKD
Document Type:
Articles
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Reprints and Research Publications, 1950-1993
SubSeries: Anfinsen Publications, 1954-1993
SubSubSeries: Numbered Reprints
Folder: 131-140
Metadata Last Modified Date:
2009-07-17
Linked Data:
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