Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus
In the late 1960s, Anfinsen's lab sought to understand the complicated details of the physicochemical properties of Staphylococcus
aureus in solution, ultimately to better understand the relationship of its structure to its function. In this article, Anfinsen,
Cuatrecasas, and Edelhoch reported that the specific binding of a competitive inhibitor resulted in a marked decrease in tyrosyl
fluorescence, which they interpreted as indicative of a specific interaction of the nucleotide with several tyrosyl groups
in the active site of the enzyme.
Number of Image Pages:
8 (896,039 Bytes)
1967-11 (November 1967)
Anfinsen, Christian B.
Periodical: Cuatrecasas, Pedro, Harold Edelhoch, and Christian B. Anfinsen. "Fluorescence Studies of the Interaction of Nucleotides
with the Active Site of the Nuclease of Staphylococcus aureus." Proceedings of the National Academy of Sciences of the
United States of America 58, 5 (November 1967): 2043-2050. Article. 8 Images.