Partial Purification of Human Interferon by Affinity Chromatography
In this article, Anfinsen, et al, reported the use of affinity chromatography to purify human leukocyte and fibroblast interferons.
By using affinity chromatography, they achieved a significant degree of purification of these interferons, but due to an insufficient
supply of starting material, a homogeneous preparation of neither interferon was obtained. Although this study was only partially
successful, until this point, efforts to purify interferon to homogeneity, which had tremendous antiviral implications, had
resulted mostly in frustration. Only later would true purification be achieved.
Item is a photocopy.
Number of Image Pages:
4 (603,250 Bytes)
1974-08 (August 1974)
Anfinsen, Christian B.
Periodical: Anfinsen, Christian B., S. Bose, Lila Corley, and D. Gurari-Rotman. "Partial Purification of Human Interferon by Affinity
Chromatography." Proceedings of the National Academy of Sciences of the United States of America 71, 8 (August 1974):
3139-3142. Article. 4 Images.
National Academy Press
Reproduced with permission of Libby Anfinsen.
Medical Subject Headings (MeSH):
Interferon and Thermophilic Bacteria, 1973-1995
Purification and Partial Characterization of Human Lymphoblastoid Interferon (November 1979)