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The Christian B. Anfinsen Papers

Title:
alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus pdf (1,010,216 Bytes) ocr (31,920 Bytes)
alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus
Description:
In this article, Anfinsen, et al, reported that while trying to study the factors that influenced protein thermostability, or the ability to remain stable at relatively high temperatures, they had cloned a gene from the hyperthermophilic bacterium Pyrococcus furiosus encoded with a highly thermostable alpha-amylase and expressed it in Escherichia Coli. The alpha-amylase expressed in E. Coli exhibited the temperature-dependent activation characteristic of the original enzyme from P. furiosus, but had a higher apparent molecular weight that was attributed to the improper formation of the native quaternary structure.
Number of Image Pages:
6 (1,010,216 Bytes)
Date:
1993-11-15 (November 15, 1993)
Creator:
Laderman, Kenneth A.
Asada, Kiyozo
Uemori, T.
Mukai, H.
Taguchi, Y.
Kato, I.
Anfinsen, Christian B.
Source:
Periodical: Laderman, Kenneth A., Kiyozo Asada, T. Uemori, H. Mukai, Y. Taguchi, I. Kato, and Christian B. Anfinsen. "alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus." Journal of Biological Chemistry 268, 32 (15 November 1993): 24402-24407. Article. 6 Images.
Publisher:
American Society for Biochemistry and Molecular Biology
Rights:
Reproduced with permission of the American Society for Biochemistry and Molecular Biology.
URL: http://www.jbc.org/Exit
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Subject:
Medical Subject Headings (MeSH):
alpha-Amylases
Pyrococcus furiosus
Recombinant Proteins
Amino Acid Sequence
Exhibit Category:
Interferon and Thermophilic Bacteria, 1973-1995
Box Number: 28
Folder Number: 7
Unique Identifier:
KKBBKP
Document Type:
Articles
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Reprints and Research Publications, 1950-1993
SubSeries: Anfinsen Publications, 1954-1993
SubSubSeries: Numbered Reprints
Folder: 221-230
Metadata Last Modified Date:
2006-06-12
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