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The Christian B. Anfinsen Papers

Title:
The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain pdf (678,486 Bytes) ocr (19,854 Bytes)
The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain
Description:
In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity appeared after the sample of bovine pancreatic ribonuclease was treated with mercaptoethanol in urea, during which period the sulfhydrl titer and the specific optical rotation changed along a curve similar to that of a first-order reaction. The lag in enzymatic activity in vitro often took several hours, while the same process seemed to take only a few minutes in vivo. This discrepancy eventually led to the discovery of an enzyme system in the endoplasmic reticulum of cells that catalyzes the disulfide interchange reaction, and subsequently, the rapid formation of the correct, native disulfide pairing in relatively short order.
Item is a photocopy.
Number of Image Pages:
6 (678,486 Bytes)
Date:
1961-09-15 (September 15, 1961)
Creator:
Anfinsen, Christian B.
Haber, Edgar
Sela, Michael
White, F. H. Jr
Source:
Periodical: Anfinsen, Christian B., Edgar Haber, Michael Sela, and F. H. White Jr. "The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain." Proceedings of the National Academy of Sciences of the United States of America 47, 9 (15 September 1961): 1309-1314. Article. 6 Images.
Publisher:
National Academy Press
Rights:
Reproduced with permission of Libby Anfinsen.
Subject:
Medical Subject Headings (MeSH):
Ribonucleases
Oxidation-Reduction
Protein Conformation
Exhibit Category:
Protein Folding and the Thermodynamic Hypothesis, 1950-1962
Box Number: 27
Folder Number: 5
Unique Identifier:
KKBBKZ
Document Type:
Articles
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Reprints and Research Publications, 1950-1993
SubSeries: Anfinsen Publications, 1954-1993
SubSubSeries: Numbered Reprints
Folder: 81-90
Metadata Last Modified Date:
2009-07-17
Linked Data:
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