The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain
In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity
appeared after the sample of bovine pancreatic ribonuclease was treated with mercaptoethanol in urea, during which period
the sulfhydrl titer and the specific optical rotation changed along a curve similar to that of a first-order reaction. The
lag in enzymatic activity in vitro often took several hours, while the same process seemed to take only a few minutes in vivo.
This discrepancy eventually led to the discovery of an enzyme system in the endoplasmic reticulum of cells that catalyzes
the disulfide interchange reaction, and subsequently, the rapid formation of the correct, native disulfide pairing in relatively
Item is a photocopy.
Number of Image Pages:
6 (678,486 Bytes)
1961-09-15 (September 15, 1961)
Anfinsen, Christian B.
White, F. H. Jr
Periodical: Anfinsen, Christian B., Edgar Haber, Michael Sela, and F. H. White Jr. "The Kinetics of Formation of Native Ribonuclease
During Oxidation of the Reduced Polypeptide Chain." Proceedings of the National Academy of Sciences of the United States
of America 47, 9 (15 September 1961): 1309-1314. Article. 6 Images.
National Academy Press
Reproduced with permission of Libby Anfinsen.
Medical Subject Headings (MeSH):
Protein Folding and the Thermodynamic Hypothesis, 1950-1962