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The Christian B. Anfinsen Papers

Title:
Disulfide Interchange and the Three-Dimensional Structure of Proteins pdf (838,322 Bytes) ocr (23,593 Bytes)
Disulfide Interchange and the Three-Dimensional Structure of Proteins
Description:
This article was published after Anfinsen's laboratory had delineated and demonstrated the major principles of protein folding and during a period when research at the lab turned to the disulfide interchange enzyme that accelerates the reactivation of the reduced form of ribonuclease. In the lab, Givol and Goldberger had worked to achieve in vitro the reactivation rates that would be compatible with the rate of protein biosynthesis. Purifying the enzyme proved difficult, so the team decided to utilize disulfide interchange activity to demonstrate other aspects related to protein folding and structure. The results of these experiments were consistent with the idea that the disulfide bonds in chymtrypsinogen were formed according to the information present in the single-chain protein, which is subsequently converted by peptide bond cleavage to the metastable three-chain chymotrypsin. Based on this, Anfinsen's group reasoned that the inactivation of a multi-chain protein by disulfide interchange indicated its origin as a single-chain protein during biosynthesis.
Item is a photocopy.
Number of Image Pages:
9 (838,322 Bytes)
Date:
1965
Creator:
Givol, David
De Lorenzo, Francesco
Goldberger, Robert
Anfinsen, Christian B.
Source:
Periodical: Givol, David, Francesco De Lorenzo, Robert Goldberger, and Christian B. Anfinsen. "Disulfide Interchange and the Three-Dimensional Structure of Proteins." Proceedings of the National Academy of Sciences of the United States of America 53, (1965): 676-684. Article. 9 Images.
Publisher:
National Academy Press
Rights:
Reproduced with permission of Libby Anfinsen.
Subject:
Medical Subject Headings (MeSH):
Protein Conformation
Protein Disulfide-Isomerases
Box Number: 27
Folder Number: 8
Unique Identifier:
KKBBLB
Document Type:
Articles
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Poor
Series: Reprints and Research Publications, 1950-1993
SubSeries: Anfinsen Publications, 1954-1993
SubSubSeries: Numbered Reprints
Folder: 111-120
Metadata Last Modified Date:
2009-07-17
Linked Data:
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