Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme
After discovering an error in their original primary sequence analysis of staphylococcal nuclease, in which residue 43 was
misidentified as glutamine instead of glutamic acid, Ontjes and Anfinsen published this paper. They reported that a resynthesis
of the correct sequence in turn yielded a product with high biological activity. Therefore, they concluded that residue 43,
although not essential for folding, was very important for catalytic activity once folding had occurred.
Number of Image Pages:
8 (946,143 Bytes)
1969-10 (October 1969)
Anfinsen, Christian B.
Periodical: Ontjes, David, and Christian B. Anfinsen. "Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease:
Properties of a Semisynthetic Enzyme." Proceedings of the National Academy of Sciences of the United States of America
64, 2 (October 1969): 428-435. Article. 8 Images.
National Academy Press
Reproduced with permission of Libby Anfinsen.
Medical Subject Headings (MeSH):
Amino Acid Sequence
The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease (October 1967)