Reductive Cleavage of Disulfide Bridges in Ribonuclease
This article was the first of many studies that were generally concerned with the eventual total synthesis of the protein.
It contained several original observations that would lead to the conclusion that the refolding of the ribonuclease molecule
after full denaturation by reductive cleavage of its four disulfide bonds required that only one of the one hundred five possible
pairings of eight sulfhydryl groups to form four disulfide linkages take place.
Number of Image Pages:
3 (322,541 Bytes)
1957-04-12 (April 12, 1957)
White, Frederick H.
Anfinsen, Christian B.
Periodical: Sela, Michael, Frederick H. White, and Christian B. Anfinsen. "Reductive Cleavage of Disulfide Bridges in Ribonuclease."
Science 125, 3250 (12 April 1957): 691-692. Article. 3 Images.
American Association for the Advancement of Science
Courtesy of Science Magazine.
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