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The Linus Pauling Papers

Title:
Properties of Antibodies pdf (266,759 Bytes) transcript of pdf
Properties of Antibodies
Description:
Item is handwritten.
Number of Image Pages:
5 (266,759 Bytes)
Date:
1941-09-26 (September 26, 1941)
Creator:
Pauling, Linus
Source:
Original Repository: Oregon State University. Library. Ava Helen and Linus Pauling Papers
Rights:
Reproduced with permission of the Ava Helen and Linus Pauling Papers. Oregon State University Library.
Subject:
Medical Subject Headings (MeSH):
Antibodies
Antigen-Antibody Complex
Exhibit Category:
How Antibodies and Enzymes Work
Unique Identifier:
MMBBCP
Document Type:
Articles
Drafts (documents)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
Properties of Antibodies
Linus Pauling
Sept. 26, 1941
During recent years there has been developed through the work of many investigations (Landsteiner, Heidelberger, Horrowitz, Marrack, Stuart Mudd, and others) a general idea of the nature of immunological process. This has been extended into a detailed picture of the structure and process of formation of antibodies by the application of our present knowledge of the structure of molecules and the nature of intermolecular interactions.
My collaborators, Dr. Dan
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Campbell, Dr. David Pressman, and Mr. Carol Iheda[?], and I have now obtained quantitative experimental results along several lines which support this picture. We have found that trivalent and trivalent haptenes, similar to those used by Landsteiner and van der Scheer, give precipitates with antibody-antigen molecular ratio close to 1. This and other results support the postulate that antibodies effective in precipitin reaction are bivalent.
Precipitates have also been obtained between antiserum
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and azoproteins with an average of only two haptenes per molecule. It has also been found that haptenes can be attached by azo groups to erythrocytes without hemolyzing the cells, and agglutination of cells has been produced with only about fifty haptenes per cell.
It was predicted that antibodies could be manufactured outside the animal by
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a process of denaturing a protein and slowly denaturing it in the presence of antigen. This has now been done, with use of a variety of denaturing agents. The dye methyl blue and an azo dye containing atoxyl have been used as antigens. The protein solutions obtained have various properties characteristic of antibodies, including specificity to the antigen used and solubility
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of antigen-antibody precipitate in excess of antigen or of haptene.
Metadata Last Modified Date:
2010-08-24
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