I thought you might be interested in the enclosed manuscript describing secondary mutations in the provirally-mutated c-myc
alleles we have cloned from bursal lymphomas. I'd be curious to know whether you've encountered any somatic mutations
in translocated c-myc alleles.
We are in the process of trying to work out some biological assay for these mutant alleles, using several cell types, including
fresh bursal cells to be returned to chemically-bursectomized birds. In view of the obvious uncertainties, we are playing
with all available technologies for getting DNA into lymphocytes; I appreciate the help we've gotten from Hunt Potter,
whose electroshock method was mentioned to me by a graduate student formerly at Harvard. If and when we obtain anything interesting
with this approach, I will let you know.