We'd like to help with your requests for antibody and src protein. However, we too have difficulty preparing large amounts
of McAB 327, and we are about to try a few other monoclonals in the immunoaffinity purification procedure. In addition, our
stocks of purified src proteins are low and prolonged storage has reduced their activity.
David Morgan, a postdoctoral fellow who has recently become a UCSF faculty member, is producing a new preparation of myristate-minus
c-src protein and would be happy to send you some of it. You can call him directly at 415/476-2251.
With best regards,
Harold E. Varmus, M.D.
American Cancer Society
Professor of Molecular Virology
P.S. Incidentally we have isolated new SH2 mutants of c-src that show strong host dependence and altered substrate preference
when isolated from avian and mammalian host cells. I'll send a preprint to you when it's ready.