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The Daniel Nathans Papers

Title:
Letter from Daniel Nathans to Bernard Weisblum pdf (57,846 Bytes) transcript of pdf
Letter from Daniel Nathans to Bernard Weisblum
Number of Image Pages:
1 (57,846 Bytes)
Date:
1973-12-11 (December 11, 1973)
Creator:
Nathans, Daniel
Recipient:
Weisblum, Bernard
University of Wisconsin. School of Medicine
Source:
Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Rights:
Reproduced with permission of Joanne Nathans.
Exhibit Category:
Restriction Enzymes and the "New Genetics," 1970-1980
Relation:
Metadata Record Letter from Bernard Weisblum to Daniel Nathans (November 16, 1973) pdf (42,244 Bytes) transcript of pdf
/ps/access/PDBBDK.pdf
Unique Identifier:
PDBBDM
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
December 11, 1973
Dear Bernie:
The gels, especially cylindrical ones, are fine. The agarose run clearly indicates that both JC and BK have short DNA, i.e., defectives. Presumably, these had not been recently plaque-purified. (We have been using agarose electrophoresis to detect and isolate deleted genomes.) This also accounts for the many minor bands after Hin A, B, C+D, E+F, G, H, I, J, K. Presumably failure to separate C and D, and E and F is due to the gradient gel; we use straight 4 percent acrylamide. The major point is well made.
For further work on the DNA, you should plaque JC and BK serially twice and pick a plaque each time from a dish containing <5 or so plaques. Grow stock from the plaque. In my experience, a plaque aspirated with a Pasteur pipette has several thousand pfu's.
With best regards,
Sincerely,
Daniel Nathans
P.S. As I mentioned to you earlier, Theresa Lee, in my lab, had also found that BK DNA (from Ken Takemoto) gives a totally different Hin digest than SV40.
Metadata Last Modified Date:
2009-12-16
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