Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Reproduced with permission of David C. Ward.
Medical Subject Headings (MeSH):
Simian virus 40
Restriction Enzymes and the "New Genetics," 1970-1980
Letter from Daniel Nathans to David C. Ward (January 25, 1971)
13th January, 1971.
Dear Dr. Nathans,
Lionel Crawford and I have recently developed a procedure for strand separation of Form II SV40 DNA which utilizes synthetic
polynucleotides as per Szybalski. We are planning to study single stranded viral DNA in a coupled in vitro protein synthesis
system and would very much like to do experiments with a unique population of linear molecules. Your recent observations with
B-restriction enzyme of E. coli suggests that it could provide such a population of DNA molecules. Would it be possible to
obtain from you a sample of the B-enzyme for use in our system. Should you be able to spare a sample we will be happy to cover
all transportation expenses.