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The Daniel Nathans Papers

Title:
Letter from David C. Ward to Daniel Nathans pdf (50,249 Bytes) transcript of pdf
Letter from David C. Ward to Daniel Nathans
Number of Image Pages:
1 (50,249 Bytes)
Date:
1971-03-16 (March 16, 1971)
Creator:
Ward, David C.
Imperial Cancer Research Fund Laboratories
Recipient:
Nathans, Daniel
Source:
Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Rights:
Reproduced with permission of David C. Ward.
Subject:
Medical Subject Headings (MeSH):
DNA Restriction Enzymes
Exhibit Category:
Restriction Enzymes and the "New Genetics," 1970-1980
Relation:
Metadata Record Letter from Daniel Nathans to David C. Ward (February 22, 1971) pdf (75,596 Bytes) transcript of pdf
/ps/access/PDBBDQ.pdf
Unique Identifier:
PDBBDR
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
16th March 1971
Dear Dr. Nathans,
Many thanks for sending the prep of restriction enzyme. It arrived in good condition a week or so ago. I've assayed it on 14C-SV40 DNA and it gave only a little less activity than you quoted, based on a 5 minute incubation. I could not, however, get detectable cleavage beyond 10 minutes. The preliminary results suggest that enzyme treated SV40 DNA will be O.K. as a starting point for strand preparation. I shall now start to prepare a large batch of the B restriction enzyme so that we can process about 100 [micro]grams of DNA. I'll let you know how things progress if the communications systems stay open (your letter of January 25th arrived today -- thanks to the postal strike). I hope I can get the project finished by June as I'm scheduled to take up my appointment at Yale in July. If you have any more information on the properties of the restriction enzyme that might be useful I'd be extremely grateful.
Thank you again for sending the enzyme.
Best personal regards.
Yours sincerely,
David C. Ward
Metadata Last Modified Date:
2009-12-18
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