Includes Nathans' handwritten response at the bottom of the letter.
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1977-02-13 (February 13, 1977)
Weizmann Institute of Science
Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Reproduced with permission of Ernest Winocour.
Restriction Enzymes and the "New Genetics," 1970-1980
February 13, 1977
As we discussed in Miami, it would be interesting to check our substituted variant which contains solely unique host DNA (coded
as CV1-1-P5 in Table 2 in Virology 73, 419, 1976) against your variant eV-1101. At the same time, it would be worthwhile reconfirming
the homology between eV-1103 host sequences and those in our CVB-1-P4 or clonal derivative. Can we interest you in a small
cooperative project on this? If so, then either we will send you the corresponding cRNA probes or, alternatively, you could
send us a few filters, each containing [approximately] 1 [micro]gram of DNA from eV-1101 and eV-1103 (this would be easier
from the point of view of the shipping). Let me know what you think.
[HANDWRITTEN NOTE: Dear Ernest,
I would be happy to cooperate in comparing host sequences in the SV40 variants we have isolated. I think the easiest thing
is to send you filter-bound DNA as you suggest. I have 1101 DNA but will need to prepare 1103.