Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Reproduced with permission of Ernest Winocour.
Medical Subject Headings (MeSH):
Simian virus 40
Restriction Enzymes and the "New Genetics," 1970-1980
November 4, 1971
Many thanks for your manuscript -- and my congratulations to you on a beautiful piece of work.
We are very interested in using the H. influenzae restriction enzyme in order to obtain information on the following point.
As you can see from the enclosed preprint (just submitted for publications, so please treat it as confidential) we now know
something about the conditions which lead to the production of closed circular SV40 DNA molecules containing sequences homologous
to host cell DNA. Obviously, we would now like to know if the homologous sequences are located at a unique site in the SV40
DNA. Maxine Singer from the NIH (she is spending a sabbatical year with us) would like to work on this problem. The plan is
to digest the special SV40 DNA molecules (containing host homologous sequences) with restriction enzyme and then, by DNA-DNA
hybridization, to see if the homologous sequences are restricted to one, or a few, of the cleavage products. Would you be
prepared to send us some H. influenzae enzyme for this purpose? Maxine and I would be happy to consider it as a co-operative
project with your group, if this fits in with your plans. If there are difficulties in shipping the enzyme in an active form,
and we have to prepare the enzyme here, we would appreciate any advice that you can give us (e.g. can the enzyme be prepared
from a non-pathogenic strain of H. influenzae, and, if so, could you send us that strain?).