Original Repository: Alan Mason Chesney Medical Archives. Daniel Nathans Collection
Reproduced with permission of Joanne Nathans.
Restriction Enzymes and the "New Genetics," 1970-1980
Letter from Janet E. Mertz to Daniel Nathans (August 9, 1974)
August 20, 1974
Thank you for the pre-prints and reprints.
In regard to the stability of deletion mutants, this varies a good deal. Ching-Ju has recloned starting with DNA recovered
from agarose, and I have re-cloned some by diluting plaque suspensions. Where there is generation of other "short genomes"
(as in the HinA deletion), they are longer than the original deleted DNA, but we don't yet know if they have duplications
or something else.