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The Sol Spiegelman Papers

Letter from Sol Spiegelman to Roy H. Doi pdf (93,716 Bytes) transcript of pdf
Letter from Sol Spiegelman to Roy H. Doi
Number of Image Pages:
1 (93,716 Bytes)
1965-07-26 (July 26, 1965)
Spiegelman, Sol
Doi, Roy H.
University of California, Davis
This item is in the public domain. It may be used without permission.
Medical Subject Headings (MeSH):
Exhibit Categories:
RNA-DNA Hybridization in Viruses, 1955-1965
Biographical Information
Metadata Record Letter from Roy H. Doi to Sol Spiegelman (July 1, 1965) pdf (68,320 Bytes) transcript of pdf
Box Number: 3
Folder Number: 1
Unique Identifier:
Document Type:
Letters (correspondence)
Physical Condition:
Series: Correspondence, 1946-1983
Folder: Doi, Roy H., 1959-1970
July 26, 1965
Dear Roy:
This is a somewhat belated reply to your letter of July 1st.
Things have been very hectic around here, the RNA problem having broken wide open and things are going along fantastically well.
Yank observed cross reactions amongst the bacteria from the very beginning. That is why we used viral and mammalian DNA to check for specificity. Subsequently, we carried out a fairly extensive examination over the past few years of this cross hybridization. This was started by Yankofsky and has been continued by David Gillespie in the bacteria and by Ritossa in higher forms. The central issue is to check whether a heterologous hybrid with ribosomal RNA actually involves the "ribosomal cistrons" of the challenging DNA. We have been using the Drosophila material as well as various strains of E. coli which we have found contain different doses of the ribosomal RNA to control this. Without this sort of information, no certain conclusions can be drawn. We will probably be writing much of this material in the near future. The trouble is we have so much because we waited until we were certain. The amount of ribosomal homology is amazing (e.g. 30% between chicken and drosophila).
I am sending you a copy of a paper which is to appear in the JMB which describes our new method of hybrid detection which is extremely accurate, sensitive, and convenient. I am also including a preprint on our RNA synthesizing system. I am just in the process of finishing up three more papers on this system. The reason why this work has suddenly blossomed forth is that we have finally been able to make infectious RNA in the test-tube in massive quantities. This gives us confidence that we are really studying the right reaction. Also, under separate cover, you will receive some more reprints of a paper given at Marseilles.
I do hope to be out on the West coast in late October since I will be attending a meeting of the Academy in Seattle. It is more than likely that I will be in and around the Bay area the week of October l8.
With kindest regards,
Sincerely yours,
S. Spiegelman
Professor of Microbiology
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