Letter from Rudolph J. Anderson to Florence R. Sabin
This letter provides another example of the ongoing collaboration between Sabin's research group and those at other institutions.
Item is handwritten.
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2 (170,498 Bytes)
1928-11-22 (November 22, 1928)
Anderson, Rudolph J.
Sabin, Florence R.
Original Repository: American Philosophical Society. Library. Florence R. Sabin Papers
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At the Rockefeller Institute for Medical Research, 1925-1938
Anderson, Rudolph J., #13
Dear Doctor Sabin,
From the chemical and physical properties of the Avian phosphatide fraction I had fully expected that it would show the same
physiological behavior as the preparation A-3 from H-37. And it is very interesting that your results show that it possesses
In regard to the sterilization of A-3 by solution and filtration before using on guinea pigs I must confess that I doubt whether
the procedure is possible at present. The solubility of the A-3 has changed somewhat since it was first isolated nearly two
years ago and it would therefore be impossible to recover the substance unchanged from an ethereal solution.
I would suggest that you sterilize by means of alcohol. For instance digest a suitable quantity of the A-3 in alcohol, in
which it is practically insoluble, until all living organisms have been killed. I don't know how long a time would be
necessary but I should think that 24 hours in alcohol would kill any organisms present. Evaporate off the alcohol at a low
temperature and preserve the residue under sterile conditions for use in the guinea pig experiments.
Of course this procedure would only kill any bacteria that are present, leaving the bacterial residues in the A-3. The amount
of such contaminating material would be very small.
After sterilization by alcohol the A-3 could be used for your experiments by dispersing it in water in the manner that you
have employed heretofore.
If this method of sterilization should not meet with your approval I believe that an aqueous suspension of the A-3 could be
sterilized by heating at a temperature of 90 [degrees] C or even at 100 [degrees] C without coagulating the suspension and
without causing any appreciable hydrolysis of the substance.