Doan worked with Sabin at the Rockefeller Institute from 1925 to 1930, and corresponded with her regularly long afterwards.
Here Sabin notes that she has sent him a hearth brush and his daughter a copy of Alice in Wonderland. She then gives a detailed
account of her studies of tubercle polysaccharides, and the effects of various controls, along with details of a new slide-fixing
technique developed by Dr. Austin Joyner.
Number of Image Pages:
2 (170,998 Bytes)
1938-02-08 (February 8, 1938)
Sabin, Florence R.
Doan, Charles A.
Original Repository: American Philosophical Society. Library. Florence R. Sabin Papers
Reproduced with permission of Geraldine F. Swan.
Medical Subject Headings (MeSH):
At the Rockefeller Institute for Medical Research, 1925-1938
Doan, Charles, #2
February 8, 1938.
Dear Doctor Doan:
At last I got the hearth brush wrapped and sent to you, and a few days ago sent the Alice in Wonderland which I hope very
much Elizabeth will enjoy.
You said you would be interested to find out what we had learned from the new study of polysaccharides. It has landed us
in a bog because we have been using all kinds of controls which do almost the same thing. We made as our standard 10 mgms.
of polysaccharide in 5 cc. saline. When you inject the 5 cc. saline alone, neutrophiles are called into the peritoneal cavity
and in 24 hours they are abundantly phagocytized by the monocytes in the milk spots. Glucose gives an even more extreme reaction
in this regard. The only difference that we have so far found with the polysaccharide, both from the tubercle bacillus and
from the pneumococcus, is that they call so many young cells from the bone marrow that in 4 hours they have actually exceeded
in number the mature neutrophiles in the blood stream. This indicates that the polysaccharide acts like the saline only more
intensely. With Type I polysaccharide from the pneumococcus we had also some stimulus of monocytes, in addition to the effect
on the neutrophilic leucocytes. In one animal with Type III pneumococcus polysaccharide the action on monocytes was even
more marked but the second animal, killed this morning, did not confirm this. Thus, as far as we have gone, almost anything
put into the peritoneal cavity will call leucocytes from the blood stream and once out into the peritoneal cavity, they are
apparently cleared away by the phagocytes. We have a new suggestion on the fixed technique which came from Mrs. Fuller in
our department and has been worked out by Doctor Joyner. It is to fix blood films, films of peritoneal exudate, and films
of omentum immediately in dioxane 25 parts and methyl (absolute) alcohol 75 parts for 1 1/2 minutes. After that, proceed
at once with the Wright stain. The advantage of the technique is that the dioxane fixes all basophilic granules, so that
the basophiles show up very clearly in the Wright stain. Doctor Joyner uses for blood films the slide method and makes the
film by taking the drop on a half slide and inverting it crossways on a whole slide, and then drawing them apart quickly as
soon as the blood has ceased spreading. He says as he draws the small slide across he lifts very slightly. At any rate,
he gets very few smudges. I think you would be interested to try this technique.
One more point has come out in our studies of the polysaccharides. We introduced the tuberculopolysaccharlde in saline without
attempting to neutralize it. The polysaccharides from the pneumococcus, on the other hand, have been given as the sodium
salt at a pH of 7.2 to 7.3 and the effects have been just the same. In following the fixed films, I am very greatly impressed
with the importance of the right pH and I wonder whether a slight acidity may not intensify an appearance of basophilia in
stained cells. One more point in technique. Slides of bone marrow prepared by the simple touch technique and fixed in dioxane-methyl
alcohol, as described above, give the most beautiful specimens of bone marrow we have ever seen. The basophile stand out
more sharply and it is possible to make, I think, quite accurate counts. The counts can be made with great rapidity because
by the touch technique the cells are all in small, isolated foci.
You will be interested to know that Doctor Cunningham is coming down this week, Saturday, to have dinner with me. He planned
to come last week but unfortunately had an attack of bronchitis. He seems to be all right now and I hope will be able to
come. I long to hear how he is getting along at Albany.
I am so well that I have entirely forgotten that I was ever sick.