Letter from Gulli Lindh Muller to Florence R. Sabin
Sabin received queries about her technique for staining living cells for many years after her initial publications on the
subject (ca. 1921). In this letter, Dr. Muller asks for advice for obtaining results similar to the ones Sabin reported.
Number of Image Pages:
2 (89,421 Bytes)
1928-06-11 (June 11, 1928)
Muller, Gulli Lindh
Sabin, Florence R.
Original Repository: American Philosophical Society. Library. Florence R. Sabin Papers
Courtesy of the American Philosophical Society.
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Medical Subject Headings (MeSH):
Staining and Labeling
On the Faculty at 'The Hopkins', 1902-1925
June 11, 1928
Dear Dr. Sabin:
Miss Randolph has not been very successful in the staining of the chick embryos. Enclosed find an account of her method.
The acetone has usually been excluded; it was tried to see whether it would improve the sections. The sections were fixed
in Bouin's fluid for one-half to one hour, according to the formula you give in your paper ("Studies of the Origin
of Blood Vessels and of Red Blood Corpuscles as Seen in the Living Blastoderm of Chick during the Second Day of Incubation").
Would you please give any suggestions you might think helpful, especially with regard to the length of time in each fluid?
The sections are not clear but have a tendency to be "muddy." Miss Randolph, who is excellent in this kind of work,
has been trying several combinations and different solutions, without entirely succeeding, and any aid you might give will
be greatly appreciated. We are working away with the chick embryos and hope to have some interesting results in the very
near future. I have had rather bad luck with the fertility of our eggs at this time of year, as most poultrymen have "torn
up their pens."
Didn't you and Dr. Doan write an article on the variation of red blood cells in the normal person? I have tried to find
the article but have not succeeded.