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The Francis Crick Papers

Title:
Letter from Francis Crick to Aaron Klug pdf (131,867 Bytes) transcript of pdf
Letter from Francis Crick to Aaron Klug
Description:
Crick here made a final comment on a draft of a paper by Klug regarding the spatial arrangement of DNA and histones in nucleosomes, particularly whether the DNA helix was itself coiled in a nucleosome (forming a coiled coil, or superhelix). Crick warned that "the whole question of the conformation of superhelical DNA in solution is full of difficulties."
In the last paragraph Crick took issue with the an alternative model of DNA presented by R. A. Rodley and colleagues in which the two strands of DNA were straight rather than helical.
Number of Image Pages:
2 (131,867 Bytes)
Date:
1977-07-01 (July 1, 1977)
Creator:
Crick, Francis
Recipient:
Klug, Aaron
Source:
Original Repository: Wellcome Library for the History and Understanding of Medicine. Francis Harry Compton Crick Papers
URL: http://archives.wellcome.ac.uk/Exit
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Rights:
Reproduced with permission of the Wellcome Library for the History and Understanding of Medicine.
URL: http://library.wellcome.ac.uk/Exit
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Subject:
Medical Subject Headings (MeSH):
DNA
Nucleosomes
Exhibit Category:
From Molecular Biology to Neurobiology, 1976-2004
Relation:
Metadata Record Letter from Aaron Klug to Francis Crick (July 18, 1977) pdf (454,357 Bytes) transcript of pdf
/ps/access/SCBBQD.pdf
Metadata Record Letter from Francis Crick to Aaron Klug (June 30, 1977) pdf (167,363 Bytes) transcript of pdf
/ps/access/SCBBQG.pdf
Metadata Record Letter from Francis Crick to Aaron Klug (June 20, 1977) pdf (207,592 Bytes) transcript of pdf
/ps/access/SCBBQH.pdf
Box Number: 23
Folder Number: PP/CRI/D/2/18
Unique Identifier:
SCBBQF
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Series: Correspondence
SubSeries: Individual Correspondents
Folder: Correspondence with Aaron Klug
Transcript:
July 1, 1977
Dear Aaron:
Thank you for your letter of 23 June and the Internal memo "Ease of twisting of DNA". Rather than reply at length could you ask Michael Levitt to show you the letter I have just written to him concerning the paper by Camerini-Otero and Felsenfeld. You will see that the whole question of the conformation of superhelical DNA in solution is full of difficulties. For example, are you sure that such DNA is not toroidal at very small [delta]L?
I don't think the variation of the sedimentation constant with [delta]L is of much use by itself. It's clear we shall need, in addition, the "thermal distribution" of [delta]L, both around a mean of [delta]L=o and also around greater values of [delta]L. We shall also need the details of the X-ray scattering in solution. On the theoretical side a lot needs to be cleared up. Hopefully a theory will eventually emerge which fits the experimental date in a natural way.
I suggest we leave further discussion till we meet in Denmark. Have you seen the preprint by Sussman and Trifonov? I am sending a copy to Michael just in case he doesn't have one.
I enclose a slightly revised version of the short note for the CSH Symposium. As you will see, it covers a number of points which we have been corresponding about. I feel that is one or two places it is sounder than the account in your paper.
Now about the New Zealand SBS structure. I mention this because you told me one of them would be visiting you in July. There seems to be no definitive papers about D loops but I am told that Kasamatsu et al. PNAS, 68, 2252-2257, 1971 is the main paper. This shows that when you unwind DNA you remove about one turn for every 10 base-pairs and certainly not one turn for every 100 base-pairs. This, by itself, should be enough to dispose of the SBS structure. Mike Botchan and Walter Keller plan to do an improved version of this experiment to see if the base-pairs/turn is nearer 10 1/2 than 10 but this will need much more careful study.
Best wishes,
F. H. C. Crick
Metadata Last Modified Date:
2010-04-27
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