Skip to main contentU.S. National Library of MedicineU.S. National Library of Medicine

Profiles in Science
Pinterest badge Follow Profiles in Science on Pinterest!

The Arthur Kornberg Papers

Letter from Arthur Kornberg to J. Oliver Lampen pdf (50,812 Bytes) transcript of pdf
Letter from Arthur Kornberg to J. Oliver Lampen
Biologists and biochemists during the 1940s and 1950s frequently shared their supplies of enzymes and other products of their labs, as there was often no other source. In this letter to J. O. Lampen, a friend from his time at the Cori lab, Kornberg regretted that he had no ready supplies of nicotinamide derivatives to send, and also discussed his current experiments.
Number of Image Pages:
1 (50,812 Bytes)
1948-10-15 (October 15, 1948)
Kornberg, Arthur
Lampen, J. Oliver
Washington University School of Medicine
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
Exhibit Category:
From Physician to Enzyme Hunter, 1942-1953
Box Number:
Unique Identifier:
Document Type:
Letters (correspondence)
Physical Condition:
October 15, 1948
Dear Joe:
I was glad to get your letter and must apologize for delaying in answering it.
I wish I had some nicotinamide derivatives to send you, but unfortunately we're rather poor at the moment. The supply of nucleotide pyrophosphatase is almost gone and I'm waiting for the arrival of Maine potatoes in order to prepare some more. The mononucleotide is obtained, as you know, by DPN hydrolysis and freed from adenylic acid and any residual DPN by Hg and Pb fractionation. It has been used in solution and has not been dried to determine its purity. We've prepared the nucleotsde by phosphatase action. There seems to be a specific but weak acid phosphatase (in prostal and potato) for this nucleotide which we've purified about 100-fold from potato extracts.
In rabbit brain where nucleosidase activity is high, the reaction seemed to be quite active on the mononucleotide as well as on DPN in a bicarbonate medium. It may be that there is nevertheless a phosphorlysis of the nucleoside.
I've been having some fun with a reaction in yeast which goes like this: Nicotinamide mononucleotide + ATP--DPN + Inorganic pyrophosphate. The equilibrium constant is about 0.5. I wish you were nearby to discuss the possible relations of this to coupled phosphorylation.
Baranowski dropped by and told me about his crystal collection and some other St. Louis highlights. It made me lonesome for the place.
Best regards,
Metadata Last Modified Date:
Linked Data:
RDF/XML     JSON     JSON-LD     N3/Turtle     N-Triples