Biologists and biochemists during the 1940s and 1950s frequently shared their supplies of enzymes and other products of their
labs, as there was often no other source. In this letter to J. O. Lampen, a friend from his time at the Cori lab, Kornberg
regretted that he had no ready supplies of nicotinamide derivatives to send, and also discussed his current experiments.
Number of Image Pages:
1 (50,812 Bytes)
1948-10-15 (October 15, 1948)
Lampen, J. Oliver
Washington University School of Medicine
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
From Physician to Enzyme Hunter, 1942-1953
October 15, 1948
I was glad to get your letter and must apologize for delaying in answering it.
I wish I had some nicotinamide derivatives to send you, but unfortunately we're rather poor at the moment. The supply
of nucleotide pyrophosphatase is almost gone and I'm waiting for the arrival of Maine potatoes in order to prepare some
more. The mononucleotide is obtained, as you know, by DPN hydrolysis and freed from adenylic acid and any residual DPN by
Hg and Pb fractionation. It has been used in solution and has not been dried to determine its purity. We've prepared the
nucleotsde by phosphatase action. There seems to be a specific but weak acid phosphatase (in prostal and potato) for this
nucleotide which we've purified about 100-fold from potato extracts.
In rabbit brain where nucleosidase activity is high, the reaction seemed to be quite active on the mononucleotide as well
as on DPN in a bicarbonate medium. It may be that there is nevertheless a phosphorlysis of the nucleoside.
I've been having some fun with a reaction in yeast which goes like this: Nicotinamide mononucleotide + ATP--DPN + Inorganic
pyrophosphate. The equilibrium constant is about 0.5. I wish you were nearby to discuss the possible relations of this to
Baranowski dropped by and told me about his crystal collection and some other St. Louis highlights. It made me lonesome for