In this letter, biochemist Alex Novikoff asked Kornberg for information about preparing Zwischenferment (the enzyme glucose-6-phosphate
dehydrogenase) and several other substances needed for the process.
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1951-05-08 (May 8, 1951)
Novikoff, Alex B.
University of Vermont
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Phyllis M. Novikoff.
Medical Subject Headings (MeSH):
From Physician to Enzyme Hunter, 1942-1953
May 8, 1951
We are getting set to try our hand at following your procedure for the preparation of Zwischenferment. When you sent me some
of your preparation some months ago, you indicated that you would be glad to send us the starting material for the enzyme
preparation. I wonder what you had in mind. If it was the Anheuser-Busch yeast, wouldn't it be possible for us to get
it directly without troubling you? In reading through the procedure I notice that aged calcium phosphate gel is used at one
point. I wonder whether you have enough of it to spare. We would like to avoid having to wait a couple of months or more
for the aging of the gel. We would be glad to send you some of our gel, in return, when it is aged.
I wonder, too, if you could send us the details of the method you use for preparing C2 and calcium phosphate gels.
Finally, I would appreciate learning your opinion of the TPN which Sigma is currently preparing. Do you know if it's
pure enough to use in your assay method for ATP?
I am sorry to impose on you so greatly. I certainly will be indebted to you for the assistance you are able to give us.