In this letter, Kornberg responded to biochemist G. David Novelli's letter describing the activity of an enzyme Kornberg
had sent him.
Number of Image Pages:
1 (43,088 Bytes)
1950-11-14 (November 14, 1950)
Novelli, G. David
Massachusetts General Hospital
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
From Physician to Enzyme Hunter, 1942-1953
Letter from G. David Novelli to Arthur Kornberg (November 6, 1950)
November 14, 1950
As you must know, I was very much interested in the information in your letter. It begins to look as if the structure of
Co A is being clarified rapidly.
I am very disappointed that the preparation we sent you had DPN splitting activity of 900 units/ml.; I am sure it started
out at 2700 units/ml. If the phosphatase contaminant in this preparation did not go down in activity--and it probably did
not--then it approaches a significant nuisance level. In our work with TPN splitting, we were careful not to prolong the
reaction beyond the time necessary to split TPN. Or the phosphate liberation was always negligible.
In paper chromatography of adenine nucleotides, ADP can be separated from the mononucleotide (as reported in a recent J. Am.
Chem. Soc. by Carter and Cohn), but I cannot tell you what the relative behavior is of the "ADP" of TPN origin.
I would be very happy to have you come down whenever it is convenient. As a formality, it may be desirable for Doctor Lipmann
to write a letter to the Director, but I am certain this will involve no difficulty at all.
With best regards to you and Doctor Lipmann from all of us,