Letter from Arthur Kornberg to the editors of the Journal of Biological Chemistry
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1958-01-29 (January 29, 1958)
Journal of Biological Chemistry
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
The Synthesis of DNA, 1953-1959
January 29, 1958.
The enclosed manuscripts have been revised to incorporate the "specific suggestions" made in your letter of January
Your letter -- last sentence of page 1 -- states that there is "no experimental evidence . . . to prove the presence of
DNA molecules not previously added as primer. The numerous editors and referees have failed to read or understand the data
in Table III (paper 2) which show a 100 per cent net synthesis of DNA, and the unequivocal statement in the text that in other
experiments net synthesis of 3 to 4 times the added DNA was observed. On the private advice of two members of the Editorial
Board, we are now adding more recent data to this table showing net increases of DNA of up to 20 times that of the added primer.
To put it another way, 90 to 95 per cent of the DNA isolated at the end of the reaction is derived from the deoxynucleoside
triphosphate substrates. This DNA is indistinguishable from DNA derived from natural sources. A statement amplifying the
one to this effect in the Discussion of paper 2 has now been appended as a footnote.
The enclosed manuscripts are in the form in which we wish to have them published. If you do not find them acceptable without
further revision, we would like to withdraw them from your consideration.