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The Arthur Kornberg Papers

Title:
Letter from Leslie E. Orgel to Arthur Kornberg pdf (39,025 Bytes) transcript of pdf
Letter from Leslie E. Orgel to Arthur Kornberg
Description:
Includes a note from Francis Crick.
Item is handwritten.
Number of Image Pages:
2 (39,025 Bytes)
Date Supplied:
ca. August 1969
Creator:
Orgel, Leslie E.
Recipient:
[Kornberg, Arthur]
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Rights:
Reproduced with permission of Leslie E. Orgel.
Subject:
Medical Subject Headings (MeSH):
Enzymes
Exhibit Category:
"Creating Life in the Test Tube," 1959-1970
Relation:
Metadata Record Letter from Arthur Kornberg to Leslie E. Orgel and Francis Crick (August 19, 1969) pdf (66,877 Bytes) transcript of pdf
/ps/access/WHBBCR.pdf
Box Number:
27
Unique Identifier:
WHBBCQ
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
Dear Arthur,
We have been discussing the Enonuclease II activity of the polymerase and have come up with the following scheme--
(a) The biological function of En II is to delete mispaired bases by a simple hydrolytic reaction. The low level of mutation is hard to understand unless one has two stages of discrimination against A-C or G-V pairing
(b) The in vitro activity against polydA-polydU is [. . .] at pH 9 because the ends of the helix are coming apart and a "loose end" looks like a mispaired base to the enzyme.
If this is true a helix of polyU against (pdA) pdG should [. . .] pdG rapidly with pH optimum clear to 7, etc. Is this worth testing?
Sincerely
Leslie Orgel
[END PAGE ONE]
[BEGIN PAGE TWO]
Unfortunately Paul has just left, so we're having to send this by post. Hope it doesn't take too long to reach you.
Francis
Metadata Last Modified Date:
2008-05-07
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