Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Leslie E. Orgel.
Medical Subject Headings (MeSH):
"Creating Life in the Test Tube," 1959-1970
Letter from Arthur Kornberg to Leslie E. Orgel and Francis Crick (August 19, 1969)
We have been discussing the Enonuclease II activity of the polymerase and have come up with the following scheme--
(a) The biological function of En II is to delete mispaired bases by a simple hydrolytic reaction. The low level of mutation
is hard to understand unless one has two stages of discrimination against A-C or G-V pairing
(b) The in vitro activity against polydA-polydU is [. . .] at pH 9 because the ends of the helix are coming apart and a "loose
end" looks like a mispaired base to the enzyme.
If this is true a helix of polyU against (pdA) pdG should [. . .] pdG rapidly with pH optimum clear to 7, etc. Is this worth
[END PAGE ONE]
[BEGIN PAGE TWO]
Unfortunately Paul has just left, so we're having to send this by post. Hope it doesn't take too long to reach you.