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The Arthur Kornberg Papers

Title:
Letter from Julius Marmur to Arthur Kornberg pdf (45,697 Bytes) transcript of pdf
Letter from Julius Marmur to Arthur Kornberg
Number of Image Pages:
1 (45,697 Bytes)
Date:
1960-01-28 (January 28, 1960)
Creator:
Marmur, Julius
Harvard University
Recipient:
Kornberg, Arthur
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Rights:
Reproduced with permission of Mildred Marmur.
Subject:
Medical Subject Headings (MeSH):
Enzymes
DNA
Escherichia coli
Exhibit Category:
"Creating Life in the Test Tube," 1959-1970
Relation:
Metadata Record Letter from I. Robert Lehman to Julius Marmur (February 9, 1960) pdf (60,105 Bytes) transcript of pdf
/ps/access/WHBBCV.pdf
Box Number:
25
Unique Identifier:
WHBBCT
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
January 28, 1960
Dear Professor Kornberg:
In the course of the isolation of high molecular weight DNA from E. coli K-12 we have encountered degradation which we believe to be due to DNAase or other nucleases. Since these preparations have been carried out in the presence of citrate and versene, it is very likely that the depolymerization is due to enzymes not requiring metal ion cofactors.
Perhaps you have had some experience with these latter enzymes in extracts of E. coli and would know how to inactivate them without degrading the DNA. I would be very grateful for any information that you could give me concerning this problem.
This work is well under way and we would be very appreciative if we could hear from you as soon as possible.
Sincerely,
Julius Marmur
Metadata Last Modified Date:
2009-02-20
Linked Data:
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