Skip to main contentU.S. National Library of MedicineU.S. National Library of Medicine

Profiles in Science
Pinterest badge Follow Profiles in Science on Pinterest!

The Arthur Kornberg Papers

Letter from Arthur Kornberg to Robert L. Sinsheimer pdf (63,557 Bytes) transcript of pdf
Letter from Arthur Kornberg to Robert L. Sinsheimer
Number of Image Pages:
1 (63,557 Bytes)
1956-12-14 (December 14, 1956)
Kornberg, Arthur
Sinsheimer, Robert L.
Iowa State College
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
Exhibit Category:
The Synthesis of DNA, 1953-1959
Box Number: 24
Folder Number: 21
Unique Identifier:
Document Type:
Letters (correspondence)
Physical Condition:
December 14, 1956
Dear Bob:
We are about to grow some cells that Zamenhof sent me in order to make a DNA of high bromo-uracil content, and we want to do two things with it:
1. Is to isolate the nucleotide, convert it to the triphosphate and see if it will be incorporated by our enzyme system.
2. We would like to use such a DNA as primer in a reaction with P32-triphosphates and then isolate from the DNAase digest of the product of such a reaction mixture a bromo-uracil containing dinucleotide. If the latter has P32 in it, then we will have evidence for a direct reaction of substrate with a nucleotide in the DNA primer.
I am writing to ask you for any advice that you can offer on the ion-exchange chromatography of the bromo-uracil nucleotide and dinucleotides. We are using your methods with great pleasure and profit.
I might mention that we are able starting with C14-labeled thymidine triphosphate to isolate C14-thymidylic dinucleotides from DNAase digests of the product. We could establish by a variety of enzymatic analyses that we have a genuine 3-5 diester, but what we can't say is whether the thymidine triphosphate reacted with deoxycytidylic (for example) as the triphosphate in the reaction mixture or as a component of the DNA. Most of our present efforts are directed toward purifying the enzyme and it has become a back-breaking logistical problem.
With many thanks for your help and very best regards,
Sincerely yours,
Arthur Kornberg
Metadata Last Modified Date:
Linked Data:
RDF/XML     JSON     JSON-LD     N3/Turtle     N-Triples