Letter from Arthur Kornberg to Robert L. Sinsheimer
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1 (63,557 Bytes)
1956-12-14 (December 14, 1956)
Sinsheimer, Robert L.
Iowa State College
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
The Synthesis of DNA, 1953-1959
Box Number: 24
Folder Number: 21
December 14, 1956
We are about to grow some cells that Zamenhof sent me in order to make a DNA of high bromo-uracil content, and we want to
do two things with it:
1. Is to isolate the nucleotide, convert it to the triphosphate and see if it will be incorporated by our enzyme system.
2. We would like to use such a DNA as primer in a reaction with P32-triphosphates and then isolate from the DNAase digest
of the product of such a reaction mixture a bromo-uracil containing dinucleotide. If the latter has P32 in it, then we will
have evidence for a direct reaction of substrate with a nucleotide in the DNA primer.
I am writing to ask you for any advice that you can offer on the ion-exchange chromatography of the bromo-uracil nucleotide
and dinucleotides. We are using your methods with great pleasure and profit.
I might mention that we are able starting with C14-labeled thymidine triphosphate to isolate C14-thymidylic dinucleotides
from DNAase digests of the product. We could establish by a variety of enzymatic analyses that we have a genuine 3-5 diester,
but what we can't say is whether the thymidine triphosphate reacted with deoxycytidylic (for example) as the triphosphate
in the reaction mixture or as a component of the DNA. Most of our present efforts are directed toward purifying the enzyme
and it has become a back-breaking logistical problem.
With many thanks for your help and very best regards,