Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
The Synthesis of DNA, 1953-1959
Letter from Arthur Kornberg to H. Gobind Khorana (February 28, 1957)
Letter from H. Gobind Khorana to Arthur Kornberg (March 18, 1957)
Box Number: 24
Folder Number: 24
March 8, 1957
I am very pleased that we can send you the enclosed sample of what I hope is terminally-labeled ATP. Sylvy prepared it with
her enzyme which appears to be free of myokinase. In an earlier experiment with P32-labeled poly-P and ADP as acceptor, P32
label was found only in the ATP. With any significant amount of myokinase some counts should have been detected in the ADP
The enclosed sample contains about 4.6 micromoles of P32-labeled ATP and about 2 micromoles of unlabeled ADP. If was prepared
by Norite adsorption and elution of Sylvy's acidified reaction mixture. There has been no chromatography and it seems
to me that for the purpose of making PRPP it would not be necessary. When counted yesterday as a dry sample under the end-window
Geiger counter (not the gas-flow, and therefore about 1/3 as sensitive as when counted on our gas-flow counter), the specific
radioactivity was of the order of 7 x 10 to the 6 counts per minute per micromole. The sample has some Norite in it which
I believe will be easily removed by centrifugation when you go to dissolve this lyophilized powder. Since we eluted the ATP
with ammoniacal ethanol I expect that it is the ammonium salt.
If you have any questions about it please to do not hesitate to call me. We are very eager to see how your results with this
ATP will compare with your earlier experiment.