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The Arthur Kornberg Papers

Title:
Letter from Arthur Kornberg to George Streisinger pdf (46,034 Bytes) transcript of pdf
Letter from Arthur Kornberg to George Streisinger
Description:
Geneticist George Streisinger was a noted expert on the bacterial virus (phage) T4. In this letter, Kornberg asked him if there might be a mutant strain of T4 suitable for studying DNA synthesis in infected E. coli cells, noting that regular T4 produced so much lysozyme that the host cells were too fragile to harvest.
Number of Image Pages:
1 (46,034 Bytes)
Date:
1965-02-02 (February 2, 1965)
Creator:
Kornberg, Arthur
Recipient:
Streisinger, George
University of Oregon
Source:
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Rights:
Reproduced with permission of Arthur Kornberg.
Subject:
Medical Subject Headings (MeSH):
DNA
Enzymes
Exhibit Category:
"Creating Life in the Test Tube," 1959-1970
Relation:
Metadata Record Letter from George Streisinger to Arthur Kornberg (February 9, 1965) pdf (155,868 Bytes) transcript of pdf
/ps/access/WHBBHG.pdf
Box Number:
32
Unique Identifier:
WHBBHF
Document Type:
Letters (correspondence)
Language:
English
Format:
application/pdf
image/tif
Physical Condition:
Good
Transcript:
February 2, 1965
Dear George:
Gerard Buttin and I have been making some attempts to study DNA synthesis in coli cells rendered permeable to triphosphates. We would like to work with T-phage-infected cells but are encountering lots of trouble with the fragility of these cells when we harvest then about 10 minutes after infection. To some extent, this fragility may be due to lysis by lysozyme and we wondered whether the use of lysozyme-defective mutants might solve this problem for us. Do you have such T4 mutants that we could try for their usefulness in this system.
Dave Hogness had the impression that you had evidence for factors or enzymes induced by phage infection that sealed the leaks in coli membranes and walls produced by phage entry or attachment. If you have references to such work or specific suggestions on how to prevent excessive leakage in infected cells, I would appreciate your letting me know.
With best personal regards,
Sincerely,
Arthur Kornberg
Metadata Last Modified Date:
2008-10-31
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