Geneticist George Streisinger was a noted expert on the bacterial virus (phage) T4. In this letter, Kornberg asked him if
there might be a mutant strain of T4 suitable for studying DNA synthesis in infected E. coli cells, noting that regular T4
produced so much lysozyme that the host cells were too fragile to harvest.
Number of Image Pages:
1 (46,034 Bytes)
1965-02-02 (February 2, 1965)
University of Oregon
Original Repository: Stanford University Libraries. Department of Special Collections and University Archives. Arthur Kornberg Papers
Reproduced with permission of Arthur Kornberg.
Medical Subject Headings (MeSH):
"Creating Life in the Test Tube," 1959-1970
Letter from George Streisinger to Arthur Kornberg (February 9, 1965)
February 2, 1965
Gerard Buttin and I have been making some attempts to study DNA synthesis in coli cells rendered permeable to triphosphates.
We would like to work with T-phage-infected cells but are encountering lots of trouble with the fragility of these cells when
we harvest then about 10 minutes after infection. To some extent, this fragility may be due to lysis by lysozyme and we wondered
whether the use of lysozyme-defective mutants might solve this problem for us. Do you have such T4 mutants that we could
try for their usefulness in this system.
Dave Hogness had the impression that you had evidence for factors or enzymes induced by phage infection that sealed the leaks
in coli membranes and walls produced by phage entry or attachment. If you have references to such work or specific suggestions
on how to prevent excessive leakage in infected cells, I would appreciate your letting me know.