Title:

Protoplasts and L-Type Growth of Escherichia coli

Creator:

Lederberg, Joshua
St. Clair, Jacqueline

Date:

February 1958

Periodical:

Journal of Bacteriology

Publisher:

American Society for Microbiology

Location:

Box: 80. Folder: 42

Rights:

Reproduced with permission of the American Society for Microbiology.

Genre:

Articles

Subject:

L Forms, Escherichia coli, Genes, Drug Resistance, Microbial, and Cell Wall

Format:

Text

Extent:

18 pages

Language:

English

Legacy Source Citation:

Periodical. Lederberg, Joshua, and Jacqueline St. Clair. "Protoplasts and L-Type Growth of Escherichia coli." Journal of Bacteriology 75, 2 (February 1958): 143-160. Article. 18 Images.. Journal of Bacteriology. Lederberg UI. P70

Legacy ID:

BBABGX

NLM ID:

101584906X832

Profiles Collection:

The Joshua Lederberg Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584906X832

Annotation:

Author: Joshua Lederberg. Date Written: October 30, 2002. KW: protoplasts; penicillin; L-forms; spheroplasts; Dienes; Klieneberger-Nobel; DAP, diaminopimelic acid; My interest in L-forms had several sources. They had played a part in pre-1946 imaginations of cellular changes in bacteria; even "zygospores" - Mellon - .. to the point that Louis Dienes had been invited to one of the Cold Spring Harbor Symposia; and I had visited Dienes in Boston. Cf also Amsterdamskaya papers re cyclogeny. Zinder & I had seen strange, filtrable forms in early interpretations of transduction; I even expanded on that in CSH 1951. That blew up of course and "FA" became phage. Nevertheless, after reading Weibull and Zinder on formation of protoplasts with lysozyme, and their stabilization with hypertonic sucrose; it came to me that penicillin-lysis might be intermediated by osmotically fragile wall-defective forms; and one Saturday afternoon experiment substantiated that. While this was the first rationally based examination of these odd forms, there remain many mysteries -- the role of agar, permanent L-forms; mg++; and what can be done with them icw cell fusion. L-forms also play some odd role in persistent, antibiotic-resistant chronic infection esp. streptococci. More: LOOKUP: exact interplay of Zinder, Weibull articles.; Correction: bibliographic reference to WORK, E. 1957 Biochemistry of the bacterial cell wall. Nature, 179, l-16. -- should be pp.841-847 The 1-16 doubtless refers to pagination of a reprint I had; DAP analogues have been explored as antimicrobials: RJ Cox and others.; jl 12/15/98; 1/24/99; 8/20/02

Lederberg Grouping:

P. Published Scientific Article.