Title:

Cell-Free Protein Synthesis Directed by Messenger RNA

Creator:

Nirenberg, Marshall W.

Date:

1964

Description:

Nirenberg's work with synthetic RNA and E. coli provided the basis for his genetic research in the early 1960s. This article describes the preparation of reagents, procedures for reaction mixtures, methods for growth of E. coli, and preparation of extracts. Their experiments ultimately proved that messenger RNA, which transcribes genetic information from DNA, directs protein synthesis. That is, messenger RNA transmits the DNA messages that prescribe the assembly of amino acids into the complex proteins that drive living processes.

Periodical:

Methods in Enzymology

Publisher:

The Academic Press

Rights:

Reproduced with permission of the Academic Press.

Genre:

Articles

Subject:

Amino Acids, RNA, Messenger, and Enzymes

Format:

Text

Extent:

7 pages

Language:

English

Legacy Source Citation:

Periodical. Nirenberg, Marshall W. "Cell-Free Protein Synthesis Directed by Messenger RNA." Methods in Enzymology VI, (1964): 17-23. Article. 7 Images.. Methods in Enzymology

Legacy ID:

JJBBHQ

NLM ID:

101584910X118

Profiles Collection:

The Marshall W. Nirenberg Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584910X118

Story Section:

Translating the Code of Life and the Nobel Prize, 1962-1968