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Summary of work for this project as indicated on the report: "Our work on muscarinic acetylcholine receptors focuses on two fundamental questions: (1) How is binding of acetylcholine to receptors converted into an intracellular response? (2) What factors regulate receptor concentrations in the membrane? A binding assay employing tritiated quinuclidinyl benzilate permits us to investigate properties of receptor binding sites and to measure receptor levels in cultured neuroblastoma and hybrid cells under systematically varied conditions."