The Dependence of Cell-Free Protein Synthesis in E. Coli Upon Naturally Occuring or Synthetic Polyribonucleotides


Title:
The Dependence of Cell-Free Protein Synthesis in E. Coli Upon Naturally Occuring or Synthetic Polyribonucleotides
Description:
In this landmark essay, published in conjunction with "Characteristics and Stabilization of DNAase-Sensitive Protein Synthesis in E. coli Extracts," the authors present the same conclusions revealed in Nirenberg's address at the now famous International Congress of Biochemistry in Moscow during August 1961. Adding an artificial form of RNA, polyuridylic acid, consisting of the base uracil, to the E. coli extract resulted in the production of an unnatural protein composed entirely of the amino acid phenylalanine. The experiment showed that UUU was the codon for phenylalanine and that messenger RNA, which transcribes genetic information from DNA, directs protein synthesis. This provided a route to base compositions of codons and the exploration of the general nature of the code.
Extent:
15 pages
Date Issued:
October 1961
Creator:
Nirenberg, Marshall W. and Matthaei, J. Heinrich (J. Heinrich Matthaei)
Source:
Periodical. Nirenberg, Marshall W., and J. Heinrich Matthaei. "The Dependence of Cell-Free Protein Synthesis in E. Coli Upon Naturally Occuring or Synthetic Polyribonucleotides." Proceedings of the National Academy of Sciences of the United States of America 47, 10 (October 1961): 1588-1602. Article. 15 Images.. Proceedings of the National Academy of Sciences of the United States of America
Periodical:
Proceedings of the National Academy of Sciences of the United States of America
Publisher:
National Academy of Sciences (U.S.)
Right Type:
Permission Granted - No Conditions
Right Statement:
Reproduced with permission of Marshall W. Nirenberg.
Subject:
RNA, RNA, Ribosomal, Escherichia coli, and RNA, Messenger
Profiles in Science ID:
JJBBDK
NLM ID:
101584910X50
Genre:
Articles
Language:
English
Format:
Text