Title:

Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver

Creator:

Goldberger, Robert E., 1937-
Epstein, Charles
Anfinsen, Christian B. (Christian Boehmer), 1916-1995

Date:

February 1963

Description:

Prior studies to this article by Anfinsen, Epstein, and Goldberger established that the time required for synthesizing a chain of protein such as ribonuclease in the tissues of higher organisms would be approximately two minutes. Here, the three researchers noted that an enzyme system in the endoplasmic reticulum of cells that catalyzed the disulfide interchange reaction and which, when added to solutions of reduced ribonuclease, catalyzed the rapid formation of the native disulfide pairing in a period less than two minutes. Subsequent studies would find that such motile proteins as staphylococcal nuclease or myoglobin could undergo virtually complete renaturation in only a few seconds or less.

Periodical:

Journal of Biological Chemistry

Publisher:

American Society for Biochemistry and Molecular Biology

Location:

Box: 27. Folder: 7

Rights:

Reproduced with permission of the American Society for Biochemistry and Molecular Biology. and http://www.jbc.org/

Genre:

Articles

Subject:

Ribonucleases, Microsomes, Oxidation-Reduction, Enzymes, and Research Design

Format:

Text

Extent:

8 pages

Relation:

Purification and Properties of a Microsomal Enzyme System Catalyzing the Reactivation of Reduced Ribonuclease and Lysozyme, 1964

Language:

English

Legacy Source Citation:

Periodical. Goldberger, Robert, Charles Epstein, and Christian B. Anfinsen. "Acceleration of Reactivation of Reduced Bovine Pancreatic Ribonuclease by a Microsomal System from Rat Liver." Journal of Biological Chemistry 238, 2 (February 1963): 628-635. Article. 8 Images.. Journal of Biological Chemistry

Legacy ID:

KKBBJX

NLM ID:

101584571X144

Profiles Collection:

The Christian B. Anfinsen Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584571X144