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The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease
- Title:
- The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease
- Creator:
- Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Corley, Lila
Ohno, Motonori
Ontjes, David A.
Eastlake, Ann - Date:
- October 1967
- Description:
- In this article, Anfinsen's team from the Laboratory of Chemical Biology at the NIAMD became the first to report that they had determined the total amino acid sequence of an extracellular nuclease of Staphylococcus aureus, which consisted of a single chain of 149 amino acid residues. They also noted that organic synthesis of the polypeptide chain of the nuclease would be of value in the direct study of catalytic and binding properties, and of factors determining tertiary structure. The proposed plan for synthesis involved assembling the chain through successive couplings of protected peptide fragments to the free alpha-amino group of protected C-terminal sequence. In this article, residue 43 was mistakenly identified as glutamine, instead of glutamic acid; A subsequent article would correct this error.
- Periodical:
- Proceedings of the National Academy of Sciences of the United States of America
- Publisher:
- National Academy Press (U.S.)
- Location:
- Box: 27. Folder: 10
- Rights:
- Reproduced with permission of Libby Anfinsen.
- Genre:
- Articles
- Subject:
- Amino Acid Sequence, Staphylococcus aureus, and Research Design
- Format:
- Text
- Extent:
- 6 pages
- Relation:
- Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme, 1969
- Language:
- English
- Legacy Source Citation:
- Periodical. Anfinsen, Christian B., David Ontjes, Motonori Ohno, Lila Corley, and Ann Eastlake. "The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease." Proceedings of the National Academy of Sciences of the United States of America 58, 4 (October 1967): 1806-1811. Article. 6 Images.. Proceedings of the National Academy of Sciences of the United States of America
- Legacy ID:
- KKBBKD
- NLM ID:
- 101584571X149
- Profiles Collection:
- The Christian B. Anfinsen Papers
- Shareable Link:
- https://profiles.nlm.nih.gov/101584571X149