Title:

Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus

Creator:

Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Edelhoch, Harold, 1922-
Cuatrecasas, Pedro

Date:

November 1967

Description:

In the late 1960s, Anfinsen's lab sought to understand the complicated details of the physicochemical properties of Staphylococcus aureus in solution, ultimately to better understand the relationship of its structure to its function. In this article, Anfinsen, Cuatrecasas, and Edelhoch reported that the specific binding of a competitive inhibitor resulted in a marked decrease in tyrosyl fluorescence, which they interpreted as indicative of a specific interaction of the nucleotide with several tyrosyl groups in the active site of the enzyme.

Periodical:

Proceedings of the National Academy of Sciences of the United States of America

Publisher:

National Academy Press (U.S.)

Location:

Box: 27. Folder: 10

Rights:

Reproduced with permission of Libby Anfinsen.

Genre:

Articles

Subject:

Binding Sites, Ribonucleases, Deoxyribonucleases, Staphylococcus aureus, and Research Design

Format:

Text

Extent:

8 pages

Language:

English

Legacy Source Citation:

Periodical. Cuatrecasas, Pedro, Harold Edelhoch, and Christian B. Anfinsen. "Fluorescence Studies of the Interaction of Nucleotides with the Active Site of the Nuclease of Staphylococcus aureus." Proceedings of the National Academy of Sciences of the United States of America 58, 5 (November 1967): 2043-2050. Article. 8 Images.. Proceedings of the National Academy of Sciences of the United States of America

Legacy ID:

KKBBKF

NLM ID:

101584571X150

Profiles Collection:

The Christian B. Anfinsen Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584571X150