Title:

Partial Purification of Human Interferon by Affinity Chromatography

Creator:

Gurari-Rotman, D.
Bose, S.
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Corley, Lila

Date:

August 1974

Description:

In this article, Anfinsen, et al, reported the use of affinity chromatography to purify human leukocyte and fibroblast interferons. By using affinity chromatography, they achieved a significant degree of purification of these interferons, but due to an insufficient supply of starting material, a homogeneous preparation of neither interferon was obtained. Although this study was only partially successful, until this point, efforts to purify interferon to homogeneity, which had tremendous antiviral implications, had resulted mostly in frustration. Only later would true purification be achieved.

Periodical:

Proceedings of the National Academy of Sciences of the United States of America

Publisher:

National Academy Press (U.S.)

Location:

Box: 28. Folder: 5

Rights:

Reproduced with permission of Libby Anfinsen.

Genre:

Articles

Subject:

Interferons, Chromatography, Affinity, and Methods

Format:

Text

Extent:

4 pages

Relation:

Purification and Partial Characterization of Human Lymphoblastoid Interferon, 1979

Language:

English

Legacy Source Citation:

Periodical. Anfinsen, Christian B., S. Bose, Lila Corley, and D. Gurari-Rotman. "Partial Purification of Human Interferon by Affinity Chromatography." Proceedings of the National Academy of Sciences of the United States of America 71, 8 (August 1974): 3139-3142. Article. 4 Images.. Proceedings of the National Academy of Sciences of the United States of America

Legacy ID:

KKBBKK

NLM ID:

101584571X154

Profiles Collection:

The Christian B. Anfinsen Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584571X154

Story Section:

Interferon and Thermophilic Bacteria, 1973-1995