The Purification and Characterization of an Extremely Thermostable alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus

Title:
The Purification and Characterization of an Extremely Thermostable alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus
Creator:
Davis, Bradley R.
Lewis, Marc S.
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Krutzsch, Henry C.
Privalov, Peter L.
Griko, Y. V.
Laderman, Kenneth A.
Date:
15 November 1993
Description:
Anfinsen believed that the discovery of hyperthermophilic bacteria in the early 1980s provided a valuable tool for the analysis of protein stability, because these bacteria made it possible to study the molecular mechanisms that governed structure and function in a system adapted for elevated temperatures. In this article, Anfinsen, et al, reported that they had purified to homogeneity the alpha-amylase, an enzyme that degrades starch, from the hyperthermophilic archaebacterium Pyrococcus furiosus. Due to the large amount of data available on alpha-amylase, Anfinsen believed that the enzyme was a "favorable" choice for the comparison of mesophilic, or optimal temperature-loving, and Thermophilic enzymes.
Periodical:
Journal of Biological Chemistry
Publisher:
American Society for Biochemistry and Molecular Biology
Location:
Box: 28. Folder: 7
Rights:
Reproduced with permission of the American Society for Biochemistry and Molecular Biology. and http://www.jbc.org/
Genre:
Articles
Subject:
Amino Acid Sequence, Enzyme Stability, and Pyrococcus furiosus
Format:
Text
Extent:
8 pages
Language:
English
Legacy Source Citation:
Periodical. Laderman, Kenneth A., Bradley R. Davis, Henry C. Krutzsch, Marc S. Lewis, Y. V. Griko, Peter L. Privalov, and Christian B. Anfinsen. "The Purification and Characterization of an Extremely Thermostable alpha-Amylase from the Hyperthermophilic Archaebacterium Pyrococcus furiosus." Journal of Biological Chemistry 268, 32 (15 November 1993): 24394-24401. Article. 8 Images.. Journal of Biological Chemistry
Legacy ID:
KKBBKN
NLM ID:
101584571X157
Profiles Collection:
The Christian B. Anfinsen Papers
Shareable Link:
https://profiles.nlm.nih.gov/101584571X157
Story Section:
Interferon and Thermophilic Bacteria, 1973-1995