Title:

Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme

Creator:

Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Ontjes, David A.

Date:

October 1969

Description:

After discovering an error in their original primary sequence analysis of staphylococcal nuclease, in which residue 43 was misidentified as glutamine instead of glutamic acid, Ontjes and Anfinsen published this paper. They reported that a resynthesis of the correct sequence in turn yielded a product with high biological activity. Therefore, they concluded that residue 43, although not essential for folding, was very important for catalytic activity once folding had occurred.

Periodical:

Proceedings of the National Academy of Sciences of the United States of America

Publisher:

National Academy Press (U.S.)

Location:

Box: 27. Folder: 13

Rights:

Reproduced with permission of Libby Anfinsen.

Genre:

Articles

Subject:

Amino Acid Sequence, Chromatography, and Peptides

Format:

Text

Extent:

8 pages

Relation:

The Synthesis of Protected Peptide Fragments of a Staphylococcal Nuclease, 1967

Language:

English

Legacy Source Citation:

Periodical. Ontjes, David, and Christian B. Anfinsen. "Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme." Proceedings of the National Academy of Sciences of the United States of America 64, 2 (October 1969): 428-435. Article. 8 Images.. Proceedings of the National Academy of Sciences of the United States of America

Legacy ID:

KKBBLH

NLM ID:

101584571X173

Profiles Collection:

The Christian B. Anfinsen Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584571X173