Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme


Title:
Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme
Description:
After discovering an error in their original primary sequence analysis of staphylococcal nuclease, in which residue 43 was misidentified as glutamine instead of glutamic acid, Ontjes and Anfinsen published this paper. They reported that a resynthesis of the correct sequence in turn yielded a product with high biological activity. Therefore, they concluded that residue 43, although not essential for folding, was very important for catalytic activity once folding had occurred.
Extent:
8 pages
Date Issued:
October 1969
Creator:
Ontjes, David A. and Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Source:
Periodical. Ontjes, David, and Christian B. Anfinsen. "Solid Phase Synthesis of a 42-Residue Fragment of Staphylococcal Nuclease: Properties of a Semisynthetic Enzyme." Proceedings of the National Academy of Sciences of the United States of America 64, 2 (October 1969): 428-435. Article. 8 Images.. Proceedings of the National Academy of Sciences of the United States of America
Periodical:
Proceedings of the National Academy of Sciences of the United States of America
Publisher:
National Academy Press (U.S.)
Right Type:
Permission Granted - No Conditions
Right Statement:
Reproduced with permission of Libby Anfinsen.
Subject:
Amino Acid Sequence, Chromatography, and Peptides
Profiles in Science ID:
KKBBLH
NLM ID:
101584571X173
Genre:
Articles
Language:
English
Format:
Text
Location:
Box: 27. Folder: 13