Title:

[Revertant DNA data] (image 2)

Creator:

Varmus, Harold

Date:

[1978-1979]

Description:

Varmus made extensive use of gel electrophoresis in his study of the genetics of retroviruses, namely to determine the quantity and structure of viral DNA prepared from extracts from the nucleus and cytoplasm (the substance surrounding the nucleus) of cells infected with Avian Sarcoma Virus (ASV). The technology enabled Varmus, for example, to show the existence of proviruses of ASV, the double-stranded DNA version of the double-stranded RNA of which the ASV genome is made. By forming a provirus, retroviruses are able to integrate themselves into the DNA of host cells.. The lanes shown in this figure were formed when electrically charged (ionic) DNA fragments, which had been dissolved in a solvent and spread on an agarose gel as a support medium, migrate across an electric field and are separated according to the speed of their migration as well as according to changes they undergo during their migration.

Original Repository:

University of California, San Francisco. Archives and Special Collections. Harold E. Varmus Papers

Location:

Box: 6. Folder: 16

Rights:

Reproduced with permission of the Regents of the University of California.

Genre:

Photographic prints

Subject:

DNA

Format:

Still Image

Extent:

1 pages

Relation:

[Revertant DNA data] (image 1), 1978
[Revertant DNA data] (image 3), 1978

Legacy Source Citation:

Original Repository. University of California, San Francisco. Archives and Special Collections. Harold E. Varmus Papers. 12290

Legacy ID:

MVBBHQ

NLM ID:

101584926X112

Profiles Collection:

The Harold Varmus Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584926X112

Story Section:

Retroviruses and the Genetic Origins of Cancer, 1970-1993