Effects of Colicins E1 and K on Cellular Metabolism
- Title:
- Effects of Colicins E1 and K on Cellular Metabolism
- Creator:
-
Luria, S. E. (Salvador Edward), 1912-1991
Fields, Kay L. - Date:
- January 1969
- Description:
- Discovered in the 1920s by Andre Gratia, colicins are water-soluble proteins that are produced by certain proteins and which kill similar kinds of bacteria. In 1963, Masayasu Nomura demonstrated that colicins kill bacteria by damaging the membrane of the bacterial cell. As a result of Nomura's work, Luria turned his focus to colicins. On leave from MIT while at the Institut Pasteur in Paris in 1963, Luria found that the particular colicin with which he worked, E1, made bacterial cells incapable of accumulating the substances they required to maintain the cell. The colicin effectively blocked the function of transport of proteins through the membrane. After he returned to MIT, the colicin work became the focus of his laboratory. Following up on their initial findings, Luria and Fields proceeded to study the fate of glucose in colicin-treated cells, determined the specific alteration of membrane permeability and found that the production of pyruvate indicated pyruvate oxidation. Their observations suggested that an early effect of these colicins on bacterial cells may be an alteration of the cytoplasmic membrane, requiring the presence of some oxygen and leading to a block in ATP-dependent processes by limiting ATP availability.
- Periodical:
- Journal of Bacteriology
- Publisher:
- American Society for Microbiology
- Rights:
- Reproduced with permission of the American Society for Microbiology. and Reproduced with permission of the American Philosophical Society.
- Genre:
- Articles
- Subject:
- Cell Membrane, Metabolism, and Colicins
- Format:
- Text
- Extent:
- 14 pages
- Language:
- English
- Legacy Source Citation:
- Periodical. Fields, Kay L., and Salvador E. Luria. "Effects of Colicins E1 and K on Cellular Metabolism." Journal of Bacteriology 97, 1 (January 1969): 64-77. Article. 14 Images.. Journal of Bacteriology
- Legacy ID:
- QLBBDN
- NLM ID:
- 101584611X53
- Profiles Collection:
- The Salvador E. Luria Papers
- Shareable Link:
- https://profiles.nlm.nih.gov/101584611X53
- Story Section:
- Biographical Information