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For this hemodynamic study, Freis's team "devised a method for determining the net bidirectional exchange of substances permeable to the capillary walls of the human forearm," using T-1824 (a dye that binds to plasma protein), sodium thiocyanate, and deuterium oxide (heavy water). They were then able to learn the rate at which water moves between body tissues and capillaries in cell metabolism.
Periodical:
Proceedings of the Society for Experimental Biology and Medicine
Periodical. Freis, Edward D., and Harold W. Schnaper. "Transcapillary Loss, Equilibrium Time, Half-Return Time of Thiocyanate and Heavy Water in the Forearm." Proceedings of the Society for Experimental Biology and Medicine 92, (1956): 188-194. Article. 7 Images.. Proceedings of the Society for Experimental Biology and Medicine