Title:

Effects of Colicins E1 and K on Transport Systems

Creator:

Luria, S. E. (Salvador Edward), 1912-1991
Fields, Kay L.

Date:

January 1969

Description:

Discovered in the 1920s by Andre Gratia, colicins are water-soluble proteins that when produced by certain proteins, can kill similar kinds of bacteria. Initially believed to be promising anti-bacterial agents, colicins soon proved to be poorly suited for the task and for decades were largely ignored by researchers. In 1963, Masayasu Nomura demonstrated that colicins are able to kill bacteria by damaging the membrane of the bacterial cell. As a result of Nomura's work, Luria turned his focus to colicins. While on leave at the Institut Pasteur in Paris in 1963, Luria found that one particular colicin, E1, made bacterial cells incapable of accumulating the substances they required to maintain the cell by effectively blocking the function of transport of proteins through the membrane. After he returned to MIT, the colicin work became the focus of his laboratory. Along with Kay Fields, Luria first looked into the possible alterations of the transport and accumulation of b-D-galactosidase--an enzyme that splits lactose into glucose and galactose--by colicin-treated E. coli cells. They found that colicin E1 drastically inhibited the energy-dependent accumulation process, while the rate of transport of ortho-nitrophenyl galactoside (ONPG)--a substrate for detection of b-galactosidase activity--was hardly affected. They further noted that the accumulation of a-methylglucoside was insensitive to colicin E1, and was thus an indication that glycolysis did proceed in colicin-inhibited cells.

Periodical:

Journal of Bacteriology

Publisher:

American Society for Microbiology

Rights:

Reproduced with permission of the American Society for Microbiology. and Reproduced with permission of the American Philosophical Society.

Genre:

Articles

Subject:

Cell Membrane and Colicins

Format:

Text

Extent:

7 pages

Language:

English

Legacy Source Citation:

Periodical. Fields, Kay L., and Salvador E. Luria. "Effects of Colicins E1 and K on Transport Systems." Journal of Bacteriology 97, 1 (January 1969): 57-63. Article. 7 Images.. Journal of Bacteriology

Legacy ID:

QLBBDM

NLM ID:

101584611X52

Profiles Collection:

The Salvador E. Luria Papers

Shareable Link:

https://profiles.nlm.nih.gov/101584611X52

Story Section:

From Phage to Colicins, 1945-1972