Studies on the Reduction and Re-formation of Protein Disulfide Bonds
The information contained in the amino acid sequence of a protein is linear, such as it is in DNA, but proteins function by
virtue of the arrangement of their linear structure into a well-defined three-dimensional conformation. In this article,
Anfinsen and Haber sought to determine how the two-dimensional data of DNA were translated into the three-dimensional information
that was characteristic of globular proteins. This simple and elegant experiment was designed to determine what would happen
when reduced, unfolded, and enzymatically inactive ribonuclease was allowed simply to refold in the presence of only neutral
aqueous buffer and air. They found that most of the activity of the enzyme was regained.
Number of Image Pages:
3 (502,149 Bytes)
1961-05 (May 1961)
Anfinsen, Christian B.
Periodical: Anfinsen, Christian B., and Edgar Haber. "Studies on the Reduction and Re-formation of Protein Disulfide Bonds." Journal
of Biological Chemistry 236, 5 (May 1961): 1361-1363. Article. 3 Images.
American Society for Biochemistry and Molecular Biology
Reproduced with permission of the American Society for Biochemistry and Molecular Biology.