It would be very hard for me to say what experiments GMAG would allow under what containment facilities. My feeling is that
cloning of so-called "cancer viruses or cancer genes" is still something that no agency will give an easy ride to
either here or in the USA. The only way to find out how GMAG thinks is submit an application to them. Their classification
of experiments as well as the experiments they consider are secret, thus I only know of my own applications and those of the
small number of applicants I am in contact with. Of all the experiments you propose I would think the cloning of the ASV
sarc minus DNA is the most probable to be approved. But I can really see no reason to use polyoma when you may well do better
in a plasmid or lambda. The MMTV genome seems to be too ill-defined both biologically and biochemically to make an argument
that a safe piece of DNA is being cloned.
I believe I told you when you were here that some years ago I started trying to clone the Herpes thymidine kinase in polyoma,
but stopped when the guidelines were published. I now have been approved by GMAG to clone mouse DNA in polyoma and one of
the genes I hope to go after, is thymidine kinase and thus see no reason to use a virus TK. Also in a country like Great
Britain there might well be resistance against using pathogenic viruses of animals (e. g., Robin has to be very careful with
VSV). But to think of it, I really don't know how pathogenic pseudorabies is.
I guess, in rereading what I have written, that I haven't been of much use. I can only suggest submitting an application
to GMAG and seeing what happens. As you know GMAG treats each case independently and if the arguments are good enough it
may well be approved in toto or with minor modification.
Please write and tell me what you would like to do next.