The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain

Title:
The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain
Creator:
Anfinsen, Christian B. (Christian Boehmer), 1916-1995
Sela, Michael
Haber, Edgar, 1932-1997
White, F. H., Jr
Date:
15 September 1961
Description:
In this article, Anfinsen, Haber, Sela, and White reported that there existed a considerable lag phase before enzymatic activity appeared after the sample of bovine pancreatic ribonuclease was treated with mercaptoethanol in urea, during which period the sulfhydrl titer and the specific optical rotation changed along a curve similar to that of a first-order reaction. The lag in enzymatic activity in vitro often took several hours, while the same process seemed to take only a few minutes in vivo. This discrepancy eventually led to the discovery of an enzyme system in the endoplasmic reticulum of cells that catalyzes the disulfide interchange reaction, and subsequently, the rapid formation of the correct, native disulfide pairing in relatively short order.
Periodical:
Proceedings of the National Academy of Sciences of the United States of America
Publisher:
National Academy Press (U.S.)
Location:
Box: 27. Folder: 5
Rights:
Reproduced with permission of Libby Anfinsen.
Genre:
Articles
Subject:
Ribonucleases, Oxidation-Reduction, and Protein Conformation
Format:
Text
Extent:
6 pages
Language:
English
Legacy Source Citation:
Periodical. Anfinsen, Christian B., Edgar Haber, Michael Sela, and F. H. White Jr. "The Kinetics of Formation of Native Ribonuclease During Oxidation of the Reduced Polypeptide Chain." Proceedings of the National Academy of Sciences of the United States of America 47, 9 (15 September 1961): 1309-1314. Article. 6 Images.. Proceedings of the National Academy of Sciences of the United States of America
Legacy ID:
KKBBKZ
NLM ID:
101584571X167
Profiles Collection:
The Christian B. Anfinsen Papers
Shareable Link:
https://profiles.nlm.nih.gov/101584571X167
Story Section:
Protein Folding and the Thermodynamic Hypothesis, 1950-1962